Detection of mRNA in Whole Mounts of Mouse Embryos Using Digoxigenin Riboprobes
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Last Update: 2020-12-08
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Source: Internet
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Author: User
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This chapter describes a nonradioactive method for the localization of mRNA in whole mouse embryos. It employs riboprobes labeled with digoxigenin, a steroid-like moiety not found in animal tissue. Digoxigenin-containing probe is visualized with a conjugate of antidigoxigenin Fab and alkaline phosphatase and colorimetric staining. The results are visualized in three dimensions, hence subtle patterns can be visualized without laborious sectioning.
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