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    Determination of 9 glucocorticoid residues in puffer fish, eel and grilled eel

    • Last Update: 2021-09-20
    • Source: Internet
    • Author: User
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    10.


    (1) Liquid chromatography conditions

    Chromatographic column: Atlantis C 18 , 3μm, 150mm×2.


    Table 10-3 Mobile phase, flow rate and gradient elution conditions



    (2) Mass spectrometry conditions

    Ion source: Electrospray ion source (ESI); Scan mode: Negative ion scan; Detection mode: Multi-reaction monitoring; Curtain gas pressure: 0.


    Table 10-4 Qualitative ion pair, quantitative ion pair, declustering voltage and collision energy of glucocorticoid

    (3) Qualitative determination

    Choose 1 parent ion and 2 product ions for each tested component.


    (4) Quantitative determination

    Quantification by external standard method



    Figure 10-19 Multiple response monitoring (MRM) chromatograms of glucocorticoids

    Related links: Determination of 9 glucocorticoid residues in puffer fish, eels and grilled eels by liquid chromatography-tandem mass spectrometry

     

     

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