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    Home > Biochemistry News > Microbiology News > Determination of Bacteriophage Genome Size by Pulsed-Field Gel Electrophoresis

    Determination of Bacteriophage Genome Size by Pulsed-Field Gel Electrophoresis

    • Last Update: 2021-02-17
    • Source: Internet
    • Author: User
    Tags

    gel electrophoresis analysis

    gel electrophoresis protocol
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    Standard agarose gel electrophoresis is extensively used to resolve
    DNA
    fragments from 0.2 to 40–50 kb. Larger fragments of genomic DNA or whole viral genomes can only effectively be resolved by pulsed-field gel electrophoresis (PFGE), which extends the range of molecular separation from 200 bp to 12 Mb.
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