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Determination of Cellular Localization of Expression of Flavin-Containing Monooxygenase Genes in Mouse Tissues by In Situ Hybridization

 Last Update: 2020-11-23
 Source: Internet
 Author: User

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Methods are described for the cellular localization of expression of flavin-containing monooxygenase (FMO) genes in various mouse tissues by
in situ
hybridization. These include the production of digoxigenin (DIG)-labeled antisense and sense RNA probes by transcription from FMO c
DNA
templates, the preparation of paraffin wax-embedded and cryostat tissue sections, the hybridization of RNA probes to tissue sections, and the specific detection of hybridized probes using an antibody to DIG.

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