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Determination of Phospholipase A2s Expression in Mast Cells by Reverse-Transcriptase Polymerase Chain Reaction

 Last Update: 2021-01-03
 Source: Internet
 Author: User

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The release of arachidonic acid (AA) from membrane phospholipids by phospholipase A
2
(PLA
2
) is a critical step in the generation of lipid mediators by mast cells. AA released by PLA
2
activity is metabolized to bioactive compounds, such as prostaglandins, thromboxane, and leukotrienes. During the last decade, several mammalian PLA2s have been shown to play a role in the mobilization of AA from phospholipids. At least ten different PLA
2
types have been described (
1
,
2
). In general, these consist of secretory, cytosolic, or membrane bound activities.

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