Determination of total antioxidant capacity in mice.

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Liu Xiaomei Song Jumin

(2006-10-24)

1, principle

has a lot of antioxidants in the body that can restore Fe3 to Fe2, which forms a solid bond with Fiorina-type substances, Its antioxidant capacity can be measured by color ratio.

2, purpose

1. Master the measurement method of total antioxidant capacity.

2. The changes in total antioxidant capacity of the blood-virtual mouse model were observed.

3. The effect of Chinese medicine on the total antioxidant capacity of blood-virtual mouse models was observed.

3, materials and methods

2. Material: EF tube (1.5ml) 120, one-time " (10ml) 60Liquid(P20ul, P100ul, P1000ul) each 2 and matching guns 200 each, glass cuvette (3ml, 1cm optical diameter) 4, warm bath box, elethromometer, vortex Vortex mixer 1 unit, ordinarycentrifuge large tube, small tube each 1, reagent bottle (125ml) 1,berries(150ml) 2,straw(10ml) 2, sucking ball 1, measure (200ml) 1, label paper 2 sheets.

3. Assay method

(1) sample processing: take the whole blood 3500 rpm / separation of the heart 15 minutes toserum to be tested.

(2) kit composition and preparation: (50T)

reagent one: liquid 60ml×2 bottles, 40C save.

Reagent II: Powder×2, with double steamed water to 120ml, stored at room temperature.

reagent three: yellow storage liquid 10 ml×1 bottle, light-safe storage. The storage fluid should be diluted 60 ml × 1 bottle.

reagent three application fluid preparation: pre-use storage fluid to dilute the dilution, the ratio is 1:19. How much preparation is required.

Reagent IV: Solution 24ml×1 bottle

reagent five: solution 24 ml×1 bottle, room temperature preservation (day cold will solidify, after each test properly warmed to accelerate dissolution, until transparent to use). - When measuring < total antioxidant capacity in > tissues,is not used when measuring serum.