Development of avian influenza vaccine for human use takes 128 days and five steps

Introduction: after more than one year's research, China has independently developed the "human avian influenza vaccine" According to the R & D unit, the clinical trial hospital for human avian influenza vaccine is still in the selection Once the pilot hospital is determined, the hospital will recruit 100 volunteers for the trial 128 days - the whole production period of human avian influenza vaccine In this production period, how is the human avian influenza vaccine born step by step? What kind of equipment is used to develop and produce this kind of vaccine that can resist the terrible virus? With these problems, our reporter visited the human avian influenza vaccine quality inspection laboratory of Beijing Science and technology company The production cycle is 128 days When the reporter arrived at Beijing Kexing at 9 a.m., he found that there were no one in the park with two football fields, and the gate of Beijing Kexing's office building was quiet But as soon as we enter Kexing, we can immediately feel the efficient and tense working state of the R & D enterprises undertaking the major national scientific research projects Put on slippers and pack your belongings As soon as the reporter walked into the human avian influenza vaccine quality inspection laboratory, he felt surrounded by white Huge heat preservation cabinet, row by row of virus cultivation tubes, strange looking "subcontracting machine" According to the "guide" of the laboratory and Ms song, director of Beijing Kexing quality inspection laboratory, the 128 days of making a human avian influenza vaccine can be roughly divided into several steps: virus cultivation, purification, sterilization, filling, subcontracting and delivery Step 1: due to confidentiality, the reporter failed to visit the process of virus culture But according to Lu Zhengyou, in order to develop the avian influenza virus vaccine, the first step is to obtain the influenza virus seed, and inoculate the seed into the healthy chicken embryo of 9-11 years old, and harvest the virus after 3 days of culture Step 2: obtain the original solution with a 12 channel micro pipette React some chemical reagents that help virus purification with the virus cultured in the test tube After a series of purification steps such as ultrafiltration, concentration and chromatography, filter out the impurities of the virus and substances harmful to human body, and purify the original solution of the vaccine Step 3: the purified vaccine stock solution will be read on the enzyme marker for vaccine detection Step 4: analyze the data, input the data read out by the enzyme marker into the computer for analysis, and master the problems in the steps of vaccine culture, ultrafiltration, concentration, chromatography, etc Step 5: the filling subcontract will analyze the correct vaccine stock solution, carry out the proportion and sterilization, obtain the vaccine semi-finished product, and then fill and sub package the stock solution to make the vaccine finished product The finished vaccine can be delivered after it has passed the self inspection of the enterprise and the verification of China Institute of pharmaceutical and biological products.