Development of Transgenic CryIA(c) + GNA Cotton Plants via Pollen Tube Pathway Method Confers Resistance to Helicoverpa armigera and Aphis gossypii Glover

Elite cotton cultivar Sumian16 was transformed with p7RPSBK-mGNA-NPTII containing
Bt
(
CryIA
(
c
)),
Galanthus nivalis
agglutinin (
GNA
) resistance genes and selectable marker
NptII
gene via the pollen tube pathway method and two fertile transgenic
Bt
+
GNA
plants were obtained in the present study. The integration and expression of the
Bt
and
GNA
genes were confirmed by molecular biology techniques and insect bioassays. Insect bioassays showed that the transformed plants were highly toxic to bollworm larvae as well as obviously retarding development of aphid populations.
PCR
analyses and identification of resistance to Kanamycin and bollworm showed that the resistance to bollworm for the two transgenic plants was dominantly inherited in a Mendelian manner and the two resistance genes and selectable marker co-segregated from primary transformed parents to the first self-fertilized progeny plants.