 Home > Biochemistry News > Plant Extracts News > Directed Evolution Through DNA Shuffling for the Improvement and Understanding of Genes and Promoters

Directed Evolution Through DNA Shuffling for the Improvement and Understanding of Genes and Promoters

 Last Update: 2020-11-15
 Source: Internet
 Author: User

Tags

rtd protein shakes

protein levels

high protein bars

Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

Unlike rational protein engineering, directed evolution provides an a priori approach toward the engineering of improved proteins and novel promoters. This minimally recursive technique builds upon small improvements by selecting and combining the best changes. Protein–protein/
DNA
interactions, catalytic efficiency, or resilience to inhibitors can be improved by thousands of times. By working within a subspace of homologous sequences, DNA shuffling recombines that subspace. Individuals are screened for a particular trait or two and selected for when they meet a set threshold. Here we explain basic principles to follow and provide procedures for the preparation, fragmentation, efficient size fractionation, and purification of parental material, as well as for the reassembly and rescue polymerase chain reactions (
PCR
s).

This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

Related Products

cas3836-23-5 Norethisterone enanthate

CAS No.: 3836-23-5

Pharmaceutical Grade

$100/KG EXW

High purity Crocetin 98% with best price 27876-94-4 and Crocetin raw powder

CAS No.: 27876-94-4

Pharmacy Grade

$8.8-10/MT FOB

Diosgenin512-04-9

CAS No.: 512-04-9

API Grade

$100/KG EXW

Hot Tags

united states jobs(223)

jobs united states(236)

1 year treasury(277)

1 year subscription(296)

single use technology(195)

announcements today(81)

ups united states(221)

united states logo(227)

80 20 company(94)

healthy food name(42)

Hot Articles

The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.