Do you know how to operate a micropipette correctly?

Micropipettes are instruments used to measure 0.
1 μl to 10 ml of liquid volumes, and are tools for sample collection and transfer during biological, chemical and clinical experiment analysis
.

According to the operation mode of pipettes, it can be divided into manual pipettes and electronic pipettes
.

Electronic pipettes are functionalized
by using motors to control the movement of the piston.

Micropipette Specification Operating Procedures 1.
Micropipette selection: Select the appropriate micropipette according to your needs
.

Usually the range is selected for operation, and the selection of this range relies less on the operator's operating skills, while ensuring the accuracy and precision
of pipetting.

2.
Adjustment of the range: Follow the principle of from large to small, when adjusting from a large number of ranges to a small range, quickly adjust to the required range by adjusting the adjustment button, and when it is close to the ideal value, the micropipette is adjusted horizontally to a predetermined value
.

When adjusting from a small scale to a large number of ranges, it is necessary to pay attention to the rotation reaching more than the predetermined value and retracing to the predetermined value
.

3.
Mounting the tip: Using the rotary mounting method, insert the micropipette end vertically into the tip, gently press hard, rotate 180 ° counterclockwise to install, do not use too much
force.

4.
Pre-wash the tip: first aspirate the sample, then drain the sample container, repeat 4 to 6 times
.

5.
Pipetting: empty the tip before washing, press the micropipette to a stop point, slowly release when sucking, do not use too much force, stay against the wall for 1 to 2 seconds
.

6.
Liquid discharge: when releasing liquid, the tip is close to the inner wall of the container and tilted 10 ° ~ 40 °, as far as possible at the bottom of the container, first press the discharge button to a stop point, after a slight pause for a second, after the remaining liquid is gathered, then press to the second stop point to press out
all the remaining liquid.

7.
Remove the tip: Lower the tip with the micropipette button and place in the container containing the
liquid.

8.
Spin the micropipette to a large number of passes
.

9.
Hang the micropipette on the
pipette holder.