Do you want antibiotics in cell culture fluid?

Why is there a call for the addition of antibiotics to cell culture fluids? Although antibiotics reduce the chance of detected contamination of microorganisms such as bacteria and yeast, they increase the chance of hidden contamination such as mycoplasma and viruses.
because pollution in cell culture is mainly caused by airborne particles and vapor mists that can carry both mycoplasma, viruses and other microorganisms.
when antibiotics are used, it can be seen that pollution is suppressed and hidden pollution is not noticed, so that the chance seditoids, viruses and other recessive pollution increases. A study by
Barile showed that 72 percent of cells cultured with antibiotics were contaminated with mycoplasma, compared with 7 percent of cell mycoplasma contamination that were not cultured with antibiotics.
in turn, if antibiotics are not used, mycoplasma and virus contamination is often accompanied by contamination of bacteria and yeast.
because of bacteria and yeast pollution is easy to observe, the timely cleaning of contaminated cells greatly reduces the chance of mycoplasma, virus pollution.
potential mycoplasma and virus pollution will bring great harm.
the presence of these microorganisms affects the physical and chemical properties of cells, allowing researchers to get the wrong results.
in severe cases, even years of research in a laboratory, wasted.
due to the improvement of filtration technology and the improvement of the quality of ultra-clean station, the chance of introducing pollution in the process of standardized cell culture operation has been greatly reduced.
it's a reasonable advice not to use antibiotics in conventional cell cultures.
use antibiotics only when there are many sources of pollution (e.g. tissue culture) or very valuable cell lines.
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