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    Home > Biochemistry News > Microbiology News > Du Wenbin's team at the Institute of Microbiology has made new progress in microfluidic high-throughput screening of plastic depolymerases

    Du Wenbin's team at the Institute of Microbiology has made new progress in microfluidic high-throughput screening of plastic depolymerases

    • Last Update: 2021-10-20
    • Source: Internet
    • Author: User
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    Plastic pollution is one of the most important environmental problems facing the world today.
    Plastic pollution will not only destroy biodiversity and exacerbate climate change, but also endanger the health of human beings and the planet
    .

    The "plastic ban" aimed at "plastic control" from the source to reduce the accumulation of plastic waste has also begun to be fully implemented throughout the country, but there is still an urgent need for efficient and environmentally friendly treatment solutions for plastics that have been exposed to the environment
    .

    Microbial degradation of plastics is the most ideal and environmentally friendly method, and it is also the focus of research in recent years
    .

    At present, many plastic depolymerizing microorganisms and enzymes from environmental and metagenomic analysis sources have been discovered, and rational and semi-rational design and transformation have been used to meet the needs of activity and thermal stability in the future
    .

    However, there are few types of microorganisms and enzymes that degrade plastics, and the degradation efficiency is low.
    Therefore, the excavation, improvement, and application development of plastic degrading enzymes are urgently needed
    .

    Du Wenbin's research group from the Institute of Microbiology, Chinese Academy of Sciences has long been committed to promoting the industrial application of microfluidic innovation technology, and applied microfluidic technology to the acquisition of environmental non-cultivable and extreme microbial resources, the screening of potential enzyme-producing and active metabolite-producing functional strains And evaluation, the ecological function and evolution of complex microbial communities, and the detection and clinical diagnosis of pathogenic microorganisms.
    Related research results were published in Angew.
    Chem.
    Int.
    Ed.
    , Anal.
    Chem.
    , Lab Chip, Environ.
    Microbiol.
    , Appl.
    .
    Environ.
    Microbiol.
    , Biosens.
    Bioelectron.
    , Small, ACS Appl.
    Mate.
    Interf.
    and other magazines
    .

    The team recently reported on the single-cell screening of plastic depolymerization microorganisms based on the microfluidic ultra-high-throughput fluorescence-activated droplet sorting platform (Fluorescence-Activated Droplet Sorting, FADS)
    .

    This work optimizes the performance of the high-throughput screening platform and the compatibility with environmental sample screening, with a screening throughput of 1000 droplets/sec and a sorting accuracy rate of over 99.
    95%
    .

    Compared with traditional screening methods, FADS provides a new platform for precise screening of a large number of microorganisms and enzyme mutants single cells
    .

    The FADS screening process consists of three steps: single cell droplet wrapping and incubation, picoliter droplet injection of PET mimic substrate fluorescein dibenzoate (FDBz), and ultra-high throughput of single cell droplets after the reaction Screening
    .

    Based on the above FADS sorting process, the team successfully obtained more than 10 strains of PET depolymerization microbial group from real wastewater samples from PET textile factories
    .

    Two new potential PET degradation enzymes that can be expressed heterologously by the engineered strains were successfully obtained from the highly active strains, and the degradation activities of the strains and the new enzymes were preliminarily verified
    .

    The follow-up team will strive to extend the FADS screening technology to the high-throughput mining and transformation of other plastics (such as PE, PP, PS, PU, ​​etc.
    ) degrading bacteria and new enzymes, so as to provide more new microorganisms for the green biodegradation and recycling of plastics.
    Resources
    .

     The above research results (Fluorescence-Activated Droplet Sorting of PET Degrading Microorganisms) were published online in the international mainstream academic journal "Journal of Hazardous Materials" (IF: 10.
    588) (doi:10.
    1016/j.
    jhazmat.
    2021.
    127417) on September 30, 2021
    .

    Dr.
    Yuxin Qiao is the first author.
    Researcher Du Wenbin from the Institute of Microbiology, Chinese Academy of Sciences and Professor Weng Yunxuan from Beijing Technology and Business University are the co-corresponding authors of the paper
    .


    The research was supported by the National Key Research and Development Program, the National Natural Science Foundation of China, the Key Frontier Science Project of the Chinese Academy of Sciences, Novozymes A/S, and the Open Project of the Key Laboratory of Green Plastic Molding Technology and Quality Evaluation in China's Light Industry.

    .


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