echemi logo
Product
  • Product
  • Supplier
  • Inquiry
    Home > Biochemistry News > Microbiology News > Dynamic Analysis of Viral Populations by Direct DNA Sequencing and Solid-Phase Technology

    Dynamic Analysis of Viral Populations by Direct DNA Sequencing and Solid-Phase Technology

    • Last Update: 2021-02-12
    • Source: Internet
    • Author: User
    Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com
    The improvements in
    DNA
    -sequencing technology in recent years has opened new possibilities, from large scale genome projects to routine diagnostic applications. Among the most important developments towards automated systems has been the introduction of the polymerase chain reaction (
    PCR
    ) and the replacement of isotopic labels by fluorescent dyes and on-line monitoring of the DNA sequence. The use of PCR for amplification of genetic material allows for direct DNA sequencing, which avoids both time-consuming cloning steps as well as control sequencing necessary to exclude “mutations” introduced by the
    Taq
    DNA polymerase (
    1
    ,
    2
    ). Thus the DNA sequence of a sample can be determined rapidly and will represent the sequence of the sample prior to amplification, as the
    Taq
    polymerase errors will not significantly contribute to the resulting signal. Different methods can be employed to obtain labeled dideoxy DNA fragments according to the Sanger methodology (
    3
    ), such as, using fluorescent-labeled primers, dideoxy chain terminators, or fluorescentlabeled dATPs. These possibilities enables many different flexible non-radioactive sequencing alternatives. In principle all commercial instruments are based on the excitation of sequencing bands by a laser beam which then can be detected in the gel during electrophoresis. An important ability with the commercially available instruments is that these enable reliable quantitation of polymorphic and heterozygous positions using different software tools (
    4
    ,
    5
    ).
    This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

    Contact Us

    The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

    Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.