Effects of FSHW waaf gene deletion on O antigen synthesis and pathogenicity of Vibrio parahaemolyticus

Vibrio parahaemolyticus is a Gram-negative halophilic bacterium that is widely distributed in marine environments and marine organisms

LPS plays an important role in the pathogenic mechanism of most Gram-negative pathogens

Dr.

Figure 1 Graphic abstract

The waaf gene deletion mutant (Δ waaf ) of O3:K6 strain (WT) was constructed by chitin-mediated transformation technology , and an apoplectic strain (C-Δ waaf ) was constructed by combining transfer method

Fig.

The results of scanning electron microscopy showed that the extracellular material of the Δwaaf

Fig.

The three strains were administered orally to mice, and the growth and survival of mice were observed

Fig.

The invasion ability of the three strains was determined by Caco-2 cells, and the results showed that the invasion of the Δwaaf strain was significantly lower than that of the other two strains; The number of Vibrio parahaemolyticus in the intestinal tract of mice with the Δwaaf strain was significantly lower than that of the other two strains, and the above results indicated that the waaf gene knockout could reduce the bacterial adhesion

Fig.

The main virulence gene ( tdh ) of Vibrio parahaemolyticus and the expression of upstream and downstream genes of waaf in the three strains were determined by fluorescence quantitative PCR .

Fig.

The waaf gene can control serotype changes in Vibrio parahaemolyticus

The first author: Zhao Feng , male, Ph.

Corresponding author: Zhou Deqing , Ph.
D.
, researcher/doctoral supervisor
.
He is currently the director of the Food Engineering and Nutrition Research Office of the Yellow Sea Fisheries Research Institute of the Chinese Academy of Fishery Sciences
.
The main research directions are aquatic product safety and quality control, aquatic product processing and comprehensive utilization, marine functional food,
etc.
In recent years, he has presided over and participated in more than 20 research projects such as the national "863" project, scientific and technological support project, and the National Natural Science Foundation of China, and trained more than 50 doctoral and master students
.
He edited and published 8 monographs, presided over the formulation and revision of more than 10 standards, and published more than 120 academic papers