Enzyme-linked immunology analysis (ELISA) of silkworm peptidease (CP)

silkworm peptidease< (CP)""FONT-WEIGHT: bold; FONT-SIZE: 14.0pt;"> enzyme-linked immuneanalysis ( ELISA )

."reagentsbox instruction manual

reagents are for researchPurpose: This kit is used to measure silkworms< in the relevant samples >span style "FONT-SIZE: 12.0pt <; COLOR: rgb (0,0,0); ><"FONT-SIZE: 12.0pt;" > content.

Experimental Principles < "FONT-WEIGHT; FONT-SIZE: FONT-SIZE: FONT-SIZE 10.5pt;">:

"this kit applicationdouble < a href" > "> antibodyclamping method assaySpecimens < "FONT-SIZE: 10.5pt;" >span Style" "FONT-SIZE:<" FONT-SIZE: 10.5pt; "> silkwormThe pyrethroidase CP) levels. With purified silkworms antibody The package < a href> "> microplate and is made into a solid phase anti < "FONT-SIZE: 10.5pt;" > body, package is single resistance >> < style: "FONT-SIZE: 10.5pt;" >, then bind to the HRP labeled pyrethroidase ( CP ) antibody to form antibodies - < a href> "> antigen - enzyme-labeled antibody complex , thoroughly washed Post plus baseTMB color. TMB at HRP enzyme catalytic conversion to blue, and under the effect of acid into the final yellow. The shade of color and the pyrethroidase (CP ) is positively related. Use an enzyme labeler to measure absorbent at 450 nm wavelengths ( OD value), through the standard curve calculated samples < "FONT-SIZE: 10.5pt;"> in the silkworm pyrethroidase (CP ) < "FONT-SIZE: 10.5pt;" > content .

kit composition < "FONT-SIZE: 10.5pt;">: