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    Home > Biochemistry News > Biotechnology News > Enzyme-linked immunoassay of rat advanced glycation end products (AGEs)

    Enzyme-linked immunoassay of rat advanced glycation end products (AGEs)

    • Last Update: 2020-11-22
    • Source: Internet
    • Author: User
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    rat lateSaccharide-based end product (AGEs < "FONT-SIZE: 14.0pt;" >-linked immunoanalytogenic

    reagents box instructions

    < class Normal"style"TEXT-INDENT: 24.0pt;">reagents are for research purposes only: This kit is used to determine rat < A href"> serum, plasma and related liquid samples in the late"color: black;" > glycosylation end product(AGEs)content.

    Experimental principle:

    test box Using < a href> "> antibody sandwich method to determine the late ratglycosylation end product(AGEs)level. With purified rat lateglycosylation end product(AGEs)antibody package ismicroplate to make solid-phase antibodies, to the packet of monoantigen micro-pores In turn, add the late span style (AGEs) ," and P." saccharide-based final product (AGEs) antibody binds to form antibodies -> <"> a href""">antigen - ase-labeled antibody complex, after thorough washing of the substrate TMB color display color. TMB is transformed into blue by the catalysis of HRP enzyme, and into the final yellow with the effect of acid. The depth of color and the late span style (AGEs) """ is positively related. Measure the absorbent at <"">450nm wavelengths" ( OD values), Using the standard curve to calculate the concentration of late rats in the sample span-based final product (AGEs ".

    kit consists :

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    kit composition

    48 < ">"

    96

    instruction manual

    <td style"BORDER-RIGHT: windowtext 1.0pt solid; PADDING-RIGHT: 5.4pt
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