Enzyme-linked immunology analysis of tissue factors (TF) in mice

< "FONT-WEIGHT: bold; FONT-SIZE: 14.0pt; COLOR: rgb (0,0,0); "> mouse tissue factor(TF)ase-immuneanalysis

< reagentsbox instruction manual

<:'FONT-SIZE: 12.0pt; COLOR: rgb (0,0,0); FONT-FAMILY: "Times New Roman"; '> reagents for research purposes onlyPurpose: This kit is used to determine the blood of mice "In , plasma and related liquid samplestissue factor (TF) < "FONT-SIZE: 12.0pt;" > content.

Experimental Principles :

Kit Application < "FONT-SIZE: 10.5pt;"> Double Antibodyclamping measurement < "FONT-SIZE: 10.5pt;"> > specimens <mouseTissue Factor (TF)level. With purified< "font-SIZE: 10.5pt; COLOR: rgb (0,0,0); "> mouseTissue Factor (TF)antibody < 'FONT-SIZE: 10.5pt; FONT-FAMILY: "Times New Roman"; '> Pack' It < a href"" > microplate and is made into a solid-phase resistance body, package is FONT-SIZE: 10.5pt; "> single-anti- span style is added to the micro-holes < FONT-SIZE: 10.5pt; COLOR: rgb (0,0,0); "> Tissue Factor (TF) , again HRP tag tissue factor (TF) antibody binds to form antibody- antigen - enzyme-labeled antibody complex , after thorough washing < "FONT-SIZE: 10.5pt;"> Ga sbrate TMB color display. TMB at HRP is catalyzed into blue and converts to final yellow by acid. The shade of color and the Tissue Factor (TF) is positively related. Font-SIZE: < 10.5pt;">450 < FONT-SIZE: 10.5pt; FONT-FAMILY: "New Times Roman"; '>nm wavelength measurement absorbent ( OD value), through the standard curve >; mouse Tissue Factor (TF) content of the water.

kit consists :