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Enzyme properties experiment - enzyme specificity

 Last Update: 2020-11-03
 Source: Internet
 Author: User

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half 1 3 4

sodium formate formula

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experimental principle

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1% casein Solution (ml)

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pH10 buffer (ml)

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distilled water (ml)

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1.0

1% starch solution (ml)

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1/2000 alkaline protein enzyme solution (ml)

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1/2000 amylase solution (ml)

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2. Shake the tubes well and keep warm for 15 minutes in a 40C water bath.
3. In tubes 3 and 4 each iodized solution 2 drops, observe the experimental phenomenon, do a good job of recording.
4. Add 3.0mL 0.4M triclosan acetic acid solution to the control tube and tube 1 and 2 respectively, shake well, filter separately, each absorb 1.0 ml of filter fluid, plus 0.4M sodium carbonate solution 5.0 ml, forin reagent 1.0 ml, shake well, keep warm for 15 minutes in a 40c water bath. Observe the phenomenon of the three tubes after removal and record them.

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