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Enzyme properties experiments - the effects of anortics and inhibitors on enzyme vitality.

 Last Update: 2020-10-25
 Source: Internet
 Author: User

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sodium hydroxide 50

yeast fermentation experiment

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"experiment principle"

Many substances can affect the catalytic activity of enzymes, substances that accelerate the catalytic action of enzymes called excitants, substances that inhibit the catalytic action of enzymes are called inhibitors. In this experiment, the agitation and inhibition of MnCl2 and HgCl2 on alkaline protease were examined respectively.

1. Experimental equipment

" and test tube racks;funnel and filter paper; < a href ""> thermostat water bath pot;thermometer; pipe pipe; 721 hydrometer

< "text-align: left;"> 2. Experiment < a href> "> reagents

(1) 1% casein solution: isoenzyme-specific experiment

(2) 1/2000 alkaline protease solution: isoenzyme-specific experiment

(3) pH10 borax sodium hydroxide buffer: isoenzyme-specific

(4) 8×10-3M MnCl2 Solution

(5)8×10-3M Hg Cl2 solution

(6) 0.4 mol/L sodium carbonate solution

(7) 0.4 mol/L III chloro acetate solution

(8) forin reagents: isoenzyme-specific experiment

take three test tubes and add reagents as follows:

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211" reagents

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1% casein solution (ml)

1.0

pH10 buffer (ml)

1.0

1.0 valt

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1.0

8×10-3M MnCl2 solution (ml)

1.0

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8× 10-3M HgCl2 solution (ml)

119"

< 1.0

distilled water (ml)

1.0

1/2000 alkaline protease solution (ml)

1.0

results

mixed evenly, in a 40c water bath for 15 minutes. Then each tube is added 0.4M trinchloro acetate solution 3.0ml, respectively,filter, each absorb filter 1.0 ml, plus 0.4M sodium carbonate solution 5.0ml, forin reagent 1.0ml, shake well, in 40 degrees C water bath insulation for 15 minutes. After coloring, a 721-type hydrometer is used to determine the light density at a wavelength of 680nm (when compared, with distilled water as a blank) and record it.

1. The activity of salivary amylase varies from person to person and sometimes varies widely, so dilution multiples can vary from person to person.

2. A small amount of agitation or inhibitor can affect the activity of the enzyme. But anthicides and inhibitors are not absolute, and some substances are an enzyme's agitant at low concentrations and inhibitors at high concentrations. One enzyme is an agitant and the other is an inhibitor.

3. Reaction time should be accurate, the utensils used in enzymatic experiments must be clean,dry , quantitatively accurate.

1. What is the most suitable temperature and pH for enzymes? What's the practical point?

2. What factors affect the catalytic activity of enzymes? Briefly explain the principle of action.

3. What conditions must the enzymatic experiment pay attention to control? Why?

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