Exploring Membrane Domains Using Native Membrane Sheets and Transmission Electron Microscopy
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Last Update: 2021-02-26
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Source: Internet
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Author: User
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The flow of information in cells requires the constant remodeling of cell signaling and trafficking networks. To observe theremodeling events associated with activation of receptors on the cell surface, the authors have generated and analyzed high-resolutiontopographical maps of colloidal gold nanoprobes (3–10 nm) marking receptors, signaling proteins, and lipids in native membranes. The technology involves sandwiching of cells between glass cover slips and electron microscopy (EM) grids, followed by ripping. Membrane sheets on EM grids are fixed, labeled with functionalized nanoprobes, and imaged by transmission electron microscopy. Probe coordinates are extracted from digitized images and the distributions of the probes are analyzed with respect to eachother and to membrane features like clathrin-coated pits, caveolae, and the cortical cytoskeleton.
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