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Expression Analysis of Jasmonate-Responsive Lectins in Plants

 Last Update: 2020-11-19
 Source: Internet
 Author: User

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recombinant protein production

expression

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The
Nicotiana tabacum
lectin, also designated Nictaba, is a nucleocytoplasmic carbohydrate-binding protein produced in tobacco leaves after application of specific jasmonates and upon insect herbivory. Here, we describe different techniques by which lectin production can be induced through exogenous jasmonate application on tobacco plants. Furthermore, we elaborate on the assays to detect
Nictaba
expression at RNA and protein levels as well as on the agglutination assays to identify the lectin activity.

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