Factors that affect migration rate in paper layer separation.

factors affecting the migration rate in paper layer separation are sample material structure, sample molecular polarity,
filtration paper
, layering solvent, PH value, temperature, mode of development and impurities in sample solution.
, sample material structure and molecular polarity: .Sample material structure and molecular polarity are the main factors affecting the migration rate.
, filter paper: .The thickness and fiber tightness of different filter paper are different, and the amount of water combined is different.impurities contained on the filter paper affect the migration rate and are pre-treated if necessary.
, layering solvent: .The migration rate of the same substance is different when it is analyzed and separated in different solvent systems. If the reagents
solvent system are
purity, they need to be pre-treated with
centrifuges and
centrifuges.
, PH value: .The pH of the solvent and sample affects the dissoluption of the substance, thus affecting the polarity and solubility of the substance and changing the migration rate.the
of the
, the water content of the flow phase increases, increasing the migration rate of polar substances. The migration rate, on the other,d.
, temperature: .Temperature affects the solubility of a substance in two phases, i.e. the distribution coefficient. Temperature affects the water cooperation of filter paper fibers, i.e. the volume of the fixed phase. In a multi-solvent system, temperature significantly affects the water content of the solvent system, i.e. the proportion of components that affect the flow phase. Therefore, the change of temperature makes the migration rate change greatly, the separation of paper
must be carried out
temperature and temperature. For some temperature-sensitive solvent systems, it is best not to be equipped with a saturated solution.
Saturday, expand the way: .The migration rate of different modes of development of the same substance is different under the same conditions of other paper layering.
, impurities in the sample solution: .Impurities in the sample solution affect the migration rate and, if necessary, are pre-treated with centrifuges.
.