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Flux Variability Analysis: Application to Developing Oilseed Rape Embryos Using Toolboxes for Constraint-Based Modeling

 Last Update: 2020-11-20
 Source: Internet
 Author: User

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Flux variability analysis enables comprehensive exploration of alternate optimal routes in a metabolic network. This method is especially useful with models such as
bna572
for the developing oilseed rape embryo which is highly compartmentalized. Here, we describe a protocol for carrying out flux variability analysis on reactions and network projections of
bna572
using well-established software (CellNetAnalyzer and COBRA) for constraint-based analysis of stoichiometric network reconstructions.

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