echemi logo
Product
  • Product
  • Supplier
  • Inquiry
    Home > Biochemistry News > Biotechnology News > Formula for commonly used buffers.

    Formula for commonly used buffers.

    • Last Update: 2020-10-21
    • Source: Internet
    • Author: User
    Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

    the formulation of a commonly used . First, the composition of the buffer Where the buffering effect of the substance is called a buffer, mostly for the weak acid and the weak acid and strong alkali made of salt, or weak alkali and the weak alkali and strong acid made of salt composition. In different proportions, with a variety of different PH buffer solution. , preparation of buffer solution notes,1. All
    reagents
    best used with secondary or first-level reagents.
    2. Sodium citrate (containing 2H2O) and citric acid (containing 2H2O) solutions are directly made with first or second grade products.
    3. Acetic acid solution, with a first or secondary ice acetic acid direct preparation, preferably with sodium hydroxide standard titration.
    4. The utensils used must be clean and the capacity instruments must be corrected to ensure accuracy.
    5. Where the standard buffer, after the distribution must go through
    acidity meter
    correction., commonly used buffer preparation1. Glycine-hydrochloric acid buffer (0.05mol/L)
    Xml 0.2 mol/L glycine plus Yml 0.2 mol/L HCl, diluted to 200ml
    2. Sodium citric acid-sodium hydroxide-hydrochloric acid buffer
    can be added to each liter of phenol, if the final change, with a small amount of 50% sodium hydroxide solution or hydrochloric acid regulation, refrigerator storage
    3. Sodium citric acid-citrate buffer (0.1 mol/L)
    C6H8O7. H2O molecular weight 210.14
    sodium citrate Na3C6H5O7.2H2O molecular weight 294.12
    4. Sodium acetate-sodium acetate buffer
    NaAc.3H2O molecular weight 136.09;
    5. Sodium dihydrophosphate -sodium dihydrophosphate buffer (0.2 mol/L)
    Na2HPO4.2H2O molecular weight 178.05
    Na2HPO4.12H2O molecular weight 358.22
    NaH2PO4. H2O molecular weight 138.01
    NaH2PO4.2H2O molecular weight 156.03
    6. Phosphate di sodium-phosphate dihydrohydrohydrohydrocarbon buffer (0.1 mol/L)
    Na2HPO4.2H2O molecular weight 178.05
    KH2PO4 molecular weight 136.09
    7. The sodium phosphate potassium dihydrohydroxide-sodium hydroxide buffer (0.05M)
    Xml 0.2 mol/L KH2PO4 plus Yml 0.2 mol/L NaOH water is diluted to 20ml.
    8. Barbiture buffer
    barbiturate sodium salt (Na diethylbarbiturate) molecular weight 206.2
    9. Trihydroxymethymeethane (Tris)-hydrochloric acid buffer (0.05 mol/L)
    50ml 0.1 mol/L trihydroxymethymethane solution is mixed with Xml 0.1 mol/L hydrochloric acid and diluted to 100ml.
    the molecular weight of trihydroxy methamethane 121.4;
    10. Boric acid-borax buffer (0.2 mol/L borate)
    borax Na2B4O7. 10H2O molecular weight 381.43
    boric acid H3BO3 molecular weight 61.84
    borax is prone to loss of crystalline water and must be stored in a plug-in bottle
    .
    This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

    Contact Us

    The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

    Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.