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Gao Caixia's latest article: Establishing a new method of wheat genome editing mediated by transient expression system

 Last Update: 2021-09-13
 Source: Internet
 Author: User

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The application of genome editing technology in plants is often restricted by the dependence on genotype and low regeneration efficiency in plant genetic transformation

Gao Caixia's research group from the Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences established a gene gun-mediated transient expression system for the GRF-GIF1 complex, which improves the regeneration ability of wheat and breaks the genotype limitation of genetic transformation, realizing efficient genome editing

The transient expression of mTaGRF4-TaGIF1 realizes an efficient wheat genome editing system that does not rely on plant genotype restrictions, and provides important technical support for wheat genome editing-mediated genetic improvement

Figure 1: Transient expression of mGRF4-GIF1 by gene bombardment can promote wheat regeneration and genome editing efficiency

Figure 2: Using mGRF4-GIF1 to edit the genome of 9 Chinese local cultivated wheat varieties

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