Generation of BAC Transgenic Mice
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Last Update: 2021-01-26
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Source: Internet
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Author: User
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Transgenic mice provide an important experimental system for studying the effects of gene overexpression and for analyzing the expression of well-characterized “single” genes that contribute to a particular trait. However, single-gene expression in transgenic (
Tg
) mice is not appropriate for the functional analysis of the many genes that may reside in a typical genetically mapped locus of the order of 1 cM. Bacterial artificial chromosome (BAC) vectors, which can accommodate large genomic intervals of up to 300 kb are appropriate for such studies (
1
). BACs of this size may contain several contiguous genes along with their
cis
-acting regulatory elements. As such, BACs are valuable tools in complementation or “rescue” experiments for identifying genes contributing to a genetically mapped quantitative trait of interest.
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