Genes that control lettuce balls have been cloned successfully for the first time

, the Proceedings of the National Academy of Sciences published a research paper by the Research Group of the Key Laboratory of the Ministry of Horticultural Plant Biology of Central China Agricultural University. The paper first reported on the cloning of the gene
that controls the cysts in lettuce, and expounded the mechanism of
's regulation of the ball in lettuce at the molecular level.
belong to the chrysanthemum family, originated in the Mediterranean region,
from wild dates, is one of the very important vegetables. The main cultivation types of dates include ball lettuce for leaves and asparagus for stems. Although the morphological differences between the different cultivation types of radon are large, the differentiation time is short and the hybrids are breedable between different types. Therefore, radon is an excellent material for studying the occurrence and evolution of plant morphology.
plant ball is a peculiar peculiar physiology of some leaf vegetables, mainly seen in the crusader's kale, cabbage, spore kale, as well as lettuce and chrysanthemum in the chrysanthemum department. These vegetable leaves are bent outwards and inwards, layered, and eventually form a spular structure consisting of leaves, called leaf balls. Ball-like has many advantages, such as brittle texture, long shelf life, easy mechanized harvesting and so on. Leaf ball is a multi-gene controlled number of sexuality, and is easily affected by the environment, which causes its genetic research difficulties, previously unsuccessful cloning of leaf ball formation of genetic regulatory genes.
the study carried out genetic analysis on F2 groups of ball lettuce and non-ball lettuce hybridization, and obtained a main effect QTL to control the ball of lettuce by the method of map cloning. The gene is a gene of the same origin
corn, the
.
tests have verified the gene's regulatory effect on the ball. In the ball lettuce, a CACTA rotell is inserted into the first exon of the gene. The insertion of the CACTA rota does not knock out the function
the gene. On the contrary, the expression of the
increased significantly after the transsego insertion. The initiation activity experiment proves that the CACTA rot constellation itself has the promoter activity.
isotopic to the KN1 gene in athropomorthic mustard, which is expressed primarily in the biogenic tissue and functions to maintain the properties of the sethicated tissue stem cells and inhibit premature differentiation of stem cells. Analysis of the genomics of dates and sunflowers
a replication of the gene in chrysanthemums. The expression patterns and
gene in radon have been significantly differentiated, and there is a higher expression in the leaves. After the gene was raised and expressed by the CACTA transstate, the near-far axis cells of the ball-ball lettuce leaves were similar, and the abdominal differentiation weakened. ChIP-seq, EMSA, and Y1H experiments have shown that
protein binds
the initiator of the gene and inhibits its expression.
expression of the blades weakens the absinth, which in turn forms the leaf ball. Over-expression in knotted lettuce
causes the blades to flip outwards, thus verifying that inhibiting the expression of the
gene is the key to the formation of the ball-like nature of lettuce.
research has been funded by the National Natural Science Foundation of China, the National Key Research and Development Program and other projects. (Source: Zhang Qingdan, China Science Journal)
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