Get out of the mistake of using biosafety cabinet and create a good operating environment

[product information of China Pharmaceutical network] the biosafety cabinet is designed to protect the operator, the experimental environment and the experimental materials when operating the infectious experimental materials such as the primary culture, bacterial strains and diagnostic specimens, so as to avoid exposure to the infectious aerosols and splashes that may be generated during the above operation When using the biosafety cabinet, there are often several misunderstandings For example, clean work table instead of biosafety cabinet I learned that in the process of use, many operators think that the biosafety cabinet and the clean work table have the same shape and function In fact, there are essential differences between them The clean working table is to blow the purified air through the filter down through the working area to protect the samples Because the working area is a positive pressure area, the air flow overflows through the operation window, only protecting the samples, not protecting the operators and the environment Biosafety cabinet is a kind of negative pressure purification platform, which can prevent operators and the environment from being exposed to harmful aerosols produced in the process of laboratory, and it is the cage of infectious microorganisms Correct operation of the biosafety cabinet can completely protect the operators, samples and working environment However, with the biosafety cabinet, it doesn't mean that everything will be OK This is actually a wrong idea In case of misoperation, leakage or damage, the biosafety cabinet cannot protect the operator "The air curtain in front of the biosafety cabinet is very vulnerable to interference and pollution." Some experts said that in the experiment, the position of the front window cannot be opened at will, the operation cannot be eased, and the arm should also avoid frequent access to the safety cabinet to prevent disturbing the air flow Not only that, operators should also establish safety awareness, develop good operation habits, put an end to illegal operation, and strictly follow the microbiology standard operating procedures and biosafety laboratory operating procedures, and use standard experimental equipment According to the industry, at present, most users don't know enough about the detection performance of the biosafety cabinet Many users think that as long as the biosafety cabinet can be turned, it will be used all the time, which has actually become a big hidden danger In fact, after the biosafety cabinet is installed, moved, repaired, and the filter is replaced, it must be retested on site In addition, users should carry out annual routine inspection and safety assessment In order to use the biosafety cabinet more safely, experts suggest that all items should be at least 2 inches away from the front window If there is any polluting operation, it should be as far away from the back of the work area as possible Clean items should be placed separately from the contaminated items The contaminated items should be placed at the back of the work area as far as possible It should be noted that during the operation of the user, objects and arms should not pass through the front window; open fire should be avoided, appropriate sterilization measures should be taken, and experimental means that interfere with the laminar flow in the safety cabinet should be avoided; when the safety cabinet is in operation state, the inner wall should be thoroughly sterilized In addition, users should do well in daily maintenance, weekly maintenance, monthly maintenance and annual maintenance For example, in terms of daily and weekly maintenance, the external surface and glass of the tank shall be cleaned with a flexible cleaner or a special cleaner for glass (it shall also be cleaned if the UV lamp is installed) In terms of monthly maintenance, it is necessary to conduct functional inspection on the equipment and check the safety of the equipment when it is normally used; in terms of annual maintenance, it is recommended that the user replace the UV lamp once a year, and the fluorescent lamp once every two years.