 Home > Biochemistry News > Microbiology News > High Isoprenoid Flux Escherichia coli as a Host for Carotenoids Production

High Isoprenoid Flux Escherichia coli as a Host for Carotenoids Production

 Last Update: 2021-02-15
 Source: Internet
 Author: User

Tags

a d1

escherichia

Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

A noncarotenogenic microbe
E. coli
was engineered for high production of carotenoids. To increase the isoprenoid flux, the chromosomal native promoters of the rate-controlling steps (
dxs, idi
and
ispDispF
) in the isoprenoid pathway were replaced with a strong bacteriophage T5 promoter (P
T5
) by using the λ-Red recombinase system in combination with the Flp/FRT site-specific recombination system for marker excision and P1 transduction for gene trait stacking. The resulting high isoprenoid flux
E. coli
can be used as a starting strain to produce various carotenoids by introducing heterologous carotenoid genes. In this study, the high isoprenoid flux
E. coli
was transformed with a plasmid carrying the β-carotene biosynthetic genes from
Pantoea stewartii
for β-carotene production
.

This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

Related Products

Fecal coliform positive control samples (multi-tube fermentation) BNCC359094 BNCC

CAS No.:

$220-280/MT EXW

Standard quality control sample of fecal coliform (multi-tube fermentation method) BNCC359289 BNCC

CAS No.:

$220-280/MT EXW

Water Quality Total Coliform Standard Quality Control Sample (Paper Rapid Method) BNCC360407 BNCC

CAS No.:

$220-280/MT EXW

Hot Tags

united states jobs(223)

jobs united states(236)

1 year treasury(277)

1 year subscription(296)

announcements today(81)

80 20 company(94)

united states logo(227)

single use technology(195)

healthy food name(42)

ups united states(221)

Hot Articles

The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.