High temperature mediates the early flowering and disease-induced memory mechanism of the amoeba.

On February 18, Cell Research published an online research paper by the Center for Excellence in Molecular Plant Sciences of the Chinese Academy of Sciences/Institute of Plant Physiology and Ecology, in collaboration with Cao Xiaofeng Research Group of the Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences, entitled An H3K27me3 demethylase-HSFA2 Loopespaespase en sie gamidosis.
the paper reveals a new mechanism for a positive feedback loop to maintain plant memory of high temperatures.
the frequent high temperature stress caused by global climate change is one of the main abiotic stresss faced by plants, which seriously affects plant growth and development and crop yield.
in order to adapt to changes in ambient temperature, plants have evolved complex genetic and epigenetic mechanisms to respond to high temperatures and adjust growth and development.
some high temperature responses can be passed on to the next generation of plants by subtracting division, and even if future generations are not affected by adversity, they still have memory markers, but the specific mechanism is unclear.
's previous research by the he Zuhua research team, through a box of immune receptorNRG1, revealed that high temperatures by reducing the sGS3 (SUPPRESSOR GENE SILENCING 3) protein content to remove genetically modified gene silencing (Posteronal Gene Silence, PTGS) and show ediphone memory effect (PNAS, 2013).
however, the specific mechanism of plant memory for high temperatures and which episophas affect plants are still unclear.
the study found that high temperatures cause early flowering of plynasis but inhibit the resistance to the Pst DC3000 (avrRpt2) of pseudomonas syringa (pseudomonas syringae) and the high temperature de-at-high seispt PTGS, which can also be passed on to the next generation, i.e. these traits have a intergenerational memory effect.
interesting, the reduction of the high temperature-mediated SGS3 protein inhibits the synthesis of ta-siRNAs and is positively correlated with the intergenerational memory of early blooming and immunosuppressive of apt.
identified an F-box ubiquitin-linked enzyme SGIP1 (SGS3-INTERACTING PROTEIN1) to be involved in the degradation of the SGS3 protein through a combination of biochemistry, molecular biology and genetics.
high temperature also has a generational memory for the upward expression of SGIP1.
in-depth study of intergenerational memory mechanisms shows that high temperature activates the thermal transcription factor HEAT SHRHSIC FACTOR A2 (HSFA2).
HSFA2, on the one hand, can directly bind to the thermal transcription element on the SGIP1 promoter to activate the expression of SGIP1, and then inhibit the production of ta-siRNAs by degradation of SGS3 protein, on the other hand, it can directly The expression of REF6 and BRM is activated by combining the heat-stimulating transcription elements on the H3K27me3 demethylate enzyme OF FLOWER FLOWER EARLYING 6 (REF6) and the chromatin remodeling factor BRAHMA (BRM) starters.
the uplift expression of REF6 and BRM can in turn reduce the H3K27me3 modification level at the HSFA 2 bit, thereby reducing the inhibition of H3K27me3 on HSFA2 transcription.
, REF6 and HSFA2 form a positive feedback loop to maintain plant memories of high temperatures.
the combination of the REF6-HSFA2 feedback loop and the ta-siRNAs synthesis decline, the expression of THE REF6/BRM/HSFA2 and ta-siRNAs common target HEAT-INDUCED TAS1 1 5 (HTT5), resulting in early flowering and increased susceptibility to plants (see figure).
the study reveals a complex epigenetic network of histonedes demethylators, chromatin remodeling factors, transcription factors, ubiquitin connectivases and small moleculeRNAs that participate in plant memory of high temperatures, prompting plants to flower early (early-knotseeds) at the expense of reducing disease resistance to ensure that plants thrive smoothly and adapt to high temperature adversity.
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He Zuhua Research Group/ Wang Yitao Research Group Postdoctoral Liu Junzhong, He Zuhua Research Group Doctoral Student Feng Lili, Gu Xuexuan, Associate Researcher of Cao Xiaofeng Research Group of the Institute of Genetics and Development, Deng Xian, co-author of the paper, He Zuhua and Cao Xiaofeng as co-authors of the newsletter.
the research provided financial support from the National Natural Science Foundation of China, the Chinese Academy of Sciences, the National Key Laboratory for Plant Molecular Genetics, the National Key Laboratory of Plant Genomics, the China Postdoctoral Science Foundation and the Youth Innovation Promotion Association of the Chinese Academy of Sciences.
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