Highly Charged Polyelectrolyte Coatings to Prevent Adsorption During Protein and Peptide Analysis in Capillary Electrophoresis
-
Last Update: 2020-12-09
-
Source: Internet
-
Author: User
Search more information of high quality chemicals, good prices and reliable suppliers, visit
www.echemi.com
Capillary electrophoresis (CE) is an interesting technique for protein and peptide analysis. However, one of the major problemsconcerns sample adsorption on the internal capillary wall. The use of non-covalent coatings using highly charged polyelectrolytesis an efficient, simple, and fast approach to reduce peptide and protein adsorption phenomena. We have studied in a systematicmanner the effect of coating conditions on the stability and efficiency of multilayer coatings using poly(diallyldimethylammonium)chloride (PDADMAC) as polycation and polystyrene sulfonate (PSS) as polyanion. When optimal conditions defined in the protocolsare used, very stable coatings are obtained and adsorption phenomena are eliminated. The coatings are stable over a largerange of pH buffer (2–10) and in the presence of organic solvent. Hundreds of analyses can be performed without coating regeneration. Coated capillaries can be easily stored and reused.
This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only.
This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of
the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed
description of the concern or complaint, to
service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content
will be removed immediately.