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Instructions for the culture of MuM-2B of human eye choroid melanoma cells
First, the cell culture conditions
| Cell name | Human eye choroid melanoma cell MuM-2B | ||
| Growth characteristics | Adherent growth | Freezing conditions | Serum-free cryopreservation solution (SKU: C7001) |
| Cultivation system | 1640+10%FBS+20mM Hepes | ||
| Generational methods | First Recommendation 1:2 Generations | Generational situation | 2-day change |
| remark | Collect the bottle medium with a sterile centrifuge tube and leave for transitional culture | ||
Second, the cells received after the treatment
of strict aseptic operation 2 (note that the sealed culture flask into the incubator should be unscrewed when the lid is loosened, after passing, it is recommended that one bottle use the original bottle of complete medium, and the other bottle uses its own complete medium in order to carry out comparative culture) Third, cell culture step
a, cell passage 2 complete medium
2
b, cell cryopreservation: cell recovery: 24, precautions:
collection of supernatant for transitional culture (later comparative culture) 25, after-sales terms:
1) Cell problems, what are the cases that can be repeated? What are the criteria for judging? 2) What are the cases in which the cells have problems and are not repeated?
