Identification of antelope horn, goat horn and sheep horn by infrared spectroscopy

Abstract: the infrared spectrum of ethanol extract from cornu antelope, cornu Caprica and Cornu ovis powder was determined by the method of evolvable potassium tablet The results showed that the infrared spectrum of ethanol extract from cornu antelope, cornu Caprica and Cornu ovis powder was obviously different, and the method was simple and accurate Keywords: antelope horn; infrared spectrum; identification antelope horn is the horn of Saiga tatarica Linnaeus It has few sources and high market price, so it belongs to precious traditional Chinese medicine In order to find a simple and reliable identification method, this paper studies the infrared fingerprint of antelope horn, goat horn and sheep horn, and finds out their differences 1 instrument and drug Perkin Elmer spectrum 20 (~ 0 fr IR spectrometer) Antelope horn (Beijing Tongrentang drugstore and Nanchang huangqingrenzhan Qingshan road drugstore) has been identified as the horn of Saiga tatarica Linnaeus, a bovine It belongs to Pharmacopoeia; goat horn (from Shandong Juye) is identified as the horn of caprahircus L of Bovinae; sheep horn (from Shandong Juye) is identified as the horn of ouisaries L of Bovinae File the above samples into fine powder for standby 2 experimental methods and results 2.1 preparation of each sample ethanol solution take 0.5g of each sample powder respectively Weigh accurately, add 20ml of ether, return to the water bath for 2h, cool down, filter, evaporate the ether residue, add 20ml of ethanol, return to the water bath for 3h, cool down, filter, wash with 20ml of ethanol, combine the filtrate, evaporate the residue in the water bath, add 2ml of 70% ethanol to dissolve the residue 2.2 determination of infrared spectrum Take 200 μ l of the above sample solutions, dry them in infrared light, add 200mg of potassium bromide powder, grind them well and compress them The infrared spectra of ethanol extracts from cornu antelope, cornu goat and Cornu sheep are measured respectively The results show that the infrared spectra of the three are significantly different 3 discussion The infrared spectrum of antelope horn measured by ethanol extract tablet pressing method can be well distinguished from that of goat horn and sheep horn: the infrared spectrum of two antelope horn samples is very similar in peak shape and peak position between 1500 / cm and 1000 / cm, and there is a sharp peak around 141l / cm; the infrared spectrum of goat horn and sheep horn has no peak, but there is a peak at 1384 / cm It has the same peak, which can be used to distinguish the antelope horn and the two Two antelope horn samples are automatically identified with the peak of 1122 / cm Although there are peaks in the infrared spectrum of goat horn and sheep horn samples, they are not identified by the instrument The results are shown in the figure According to the spectrum, we can choose the absorbance ratio a (1411 / cm) between 141l / cm and 1384 / cm ）/ a (1384 / cm), using the different ratio to distinguish antelope horn, goat horn and sheep horn.