Immun Inflamm Dis: The functional phenotype of DENV-specific T cells is associated with the severity of dengue fever.

Although the role of DENV-specific T cells in the pathogenesis of acute dengue infection has become clear, the function of viral-specific T cells associated with less severe clinical diseases is not yet clear!---- The purpose of this study was to explore the differences in the function of DENV-NS3 and DENV-NS5 protein-specific T cells in patients with dengue fever (DF) and dengue haemorrhagic fever (DHF)Using intracellular cytokine testing, we evaluated the production of interferon (IFN) in adult patients with acute DF (n s 21) and DHF (n s 22), tumor necrosis factor-alpha (TNF-alpha), macrophage inflammatory protein-1 beta (MIP-1 beta) and the expression of CD107aThe results showed that dF patients produced four cytokines, multi-function DENV-NS3- and DENV-NS5-specific T cells more frequently than dHF patientsDenV-NS3-and DENV-NS5-specific T cells in DF patients express IFN-and-TNF-alpha, MIP-beta,CD107a, while T-cells in DHF patients are mainly expressed for CD107a and MIP-1In DF patients, there was a significant increase in the overall production of IFN-sodium or TNF-alpha in DENV-NS3-and DENV-NS5-specific T cellsMost NS3-specific T cells in DF (78.6%) and DHF (68.9%) patients are the creators of single cytokines, and 76.6% of DENV-NS5-specific T cells in DF patients and 77.1% of T cells in DHF patients produce only single cytokinesHowever, no significant association was found with multi-functional T-cell reactions and viral haemorrhageIn summary, the results show that the functional phenotype of DENV-specific T cells is likely to be related to the severity of clinical diseases