Important progress has been made in the research on the impact of organic pollutants on the blood-brain barrier

Researchers from the Environmental Toxicology Discipline Group of the Institute of Hydrobiology, Chinese Academy of Sciences use human brain microvascular endothelial cells as the in vitro blood-brain barrier model, and the transgenic zebrafish ( TG flk1 : EGFP ) provided by the National Zebrafish Resource Center as the living blood-brain barrier model , new contaminants tetrabromophthalate ester bis ( bIS (2-ethylhexyl) -3,4,5,6-tetrabromophthalate, TBPH ) as the object of study, the blood-brain barrier and its ability to penetrate the blood-brain of The impact of barrier structure and function, and the resulting neurotoxic effects were evaluated

The results from the study showed that body, TBPH to 6 approximately hours Tanswell reached equilibrium chamber on both sides of the substrate, which is far below the apparent permeability coefficient reference fluorescein sodium ( NAFL ), showed TBPH lower ability to penetrate the blood brain barrier ; But TBPH can increase the permeability of the blood-brain barrier without affecting cell viability (Figure 1 )

Figure 1 The ability of TBPH to cross the blood-brain barrier and its effect on the viability and function of hCMEC/D3 cells

(A) The effect of TBPH on the viability of hCMEC/D3 cells; (B) The content of TBPH outside of the Transwell chamber ; (C) The permeability of TBPH and sodium fluorescein; (D) TBPH passes through the sodium fluorescein within 6 h The effect of hCMEC/D3 cell permeability .

Further in vivo studies have found that TBPH can increase the permeability of the zebrafish blood-brain barrier, which may be due to the fact that TBPH can interact with tight junction proteins, thereby affecting its structural integrity

Figure 2.