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With the development of next-generation sequencing technology and the declining cost of Whole Genome Sequencing (WGS), WGS-based Bulk Segregation Analysis (BSA) has become a routine tool for quickly locating candidate genes.
Feng Xianzhong's team at the Key Laboratory of Soybean Molecular Design and Breeding, Northeast Institute of Geography and Agricultural Ecology, Chinese Academy of Sciences, reported an improved version of WGS-BSA method called M2-seq around the functional gene location of soybean mutants.
This study was published in the international journal Frontiers in Plant Science with the title "A robust and rapid candidate gene mapping pipeline based on M2 populations".