In Situ PCR Amplification of Intracellular mRNA
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Last Update: 2021-02-26
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Source: Internet
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Author: User
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The polymerase chain reaction (
PCR
) is now commonly used in laboratories involved in research studies and clinical diagnostic work (
1
,
2
). A major advantage of PCR combined with reverse transcription (
RT-PCR
) is that it can be used to amplify and detect rare mRNA within a specimen. However, conventional RT-PCR cannot be used either to quantitate the frequency of cells expressing a particular mRNA or determine the cellular origin of the amplified signal. Both of these factors may be relevant in the interpretation of gene expression.
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