Inherent drug-resistant gene research: the horizontal transfer mechanism of the ant(3")-II gene in immobilized bacteria.

Resistance to bacillus includes access resistance and inherent resistance.
is a natural tolerance of bacteria to certain types of antibiotics, determined by the inherent resistance gene.
-resistant genes are those that are conservatively positioned on the chromosomes of certain bacteria and are drug-resistant.
the discovery of inherent drug-resistant genes could provide a target for new drug development, and inherent drug-resistant genes could be captured by moving originals as a source of obtained resistance.
For inherent drug resistance, the Feng Wei Team of the Institute of Microbiology of the Chinese Academy of Sciences has previously discovered a new type of intrinsic large-ring esterphosphate transferase in wax-like spores and copper-green protons, respectively. 5): 1560-73) and the inherent amino glycoside phosphate transferase resistance gene (Antimicrob Agents Chemother.2016, 60 (11): 6983-85).
Recently, Feng's team found a new type of amino glycoside adenosine transferase ANT(3"-II) in the human-important pathogenic bacteria Immobile, which is widely distributed in the genus IIa, IIb, IIc, including the clinically common Acinetototer baumanii (IBacate baumanii, I Ia), Acinetobacter Pittii, IIa, Ainetobacter gyllenbergii, Acinetobacter parvus, IIc, and other undetermined immobile bacteria (Acinetobacter sp., IIb and IIc).
system evolution analysis shows that ANT(3")-II's evolutionary relationship with the known ANT(3")-I has recently formed two separate branches in the evolutionary tree, with the known ANT(3"-I generally located in the Gloches The moving originals of the bacteria, while ANT(3")-II is specific to the conservative position of the chromosomes of Bacillus, so ANT(3")-II is a new amino glycoside adenosine transfer enzyme subsome.
further studies have found that the ant(3")-II gene can be transferred frequently at a level in immobilized bacteria.
e.g. the ant(3")-IIa gene of Baumann's Psychic bacteria can be transferred to Acinetobacter junii, and this horizontal transfer is mediated by homicrobial recombination rather than mobile originals (Figure 1).
Recombinant hot spot analysis of all genes contained in Bacillus do not move found that the ant(3")-II gene is located in the recombinant hot spot of chromosomes (Figure 2), indicating that the ant(3)-II gene is frequently transferred in Bacillus in motion.
findings suggest for the first time that inherent drug-resistant genes can be transmitted between different strains of immobilized bacteria by cogeneration.
paper, entitled A new subclass of intrinsic aminoglycoside nucleotidyltransferases, ANT (3")-II, horizontally transferred among acinetobacter spp.by homologous recombination, was published online in February 2017 on Plos Genetics.
, an assistant researcher at the Institute of Microbiology, is the first author of the paper and Feng Wei is the author of the paper.
research was funded by the National Key Basic Research and Development Program ("973") and the National Natural Science Foundation.
.