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    Home > Biochemistry News > Microbiology News > Insect Cell- Culture Techniques in Serum- Containing Medium

    Insect Cell- Culture Techniques in Serum- Containing Medium

    • Last Update: 2021-02-22
    • Source: Internet
    • Author: User
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    Almost 50 years ago, Grace reported the successful establishment of continuous cell lines from insects that were without defined biological and biophysical parameters (
    1
    ). Since that time, the minimal distinctive parameters for the establishment, maintenance, and manipulation of invertebrate cell cultures have been identified and optimized (
    2
    ,
    3
    ). The establishment of new lines is now routine, and the culture of hundreds of cell lines from a variety of insect species have to date been reported (
    2
    ,
    4
    ,
    5
    ,
    6
    ). During the last 10 years, genetic engineering has revolutionized insect cell culture and baculo virology; this technology has become the method of choice for production of a variety of recombinant proteins of medical and agricultural importance (
    7
    ,
    8
    ). These scientific events have renewed previous interest in using cell culture for production of baculovirus for viral pesticides (
    9

    14
    ).
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