Insect Cell- Culture Techniques in Serum- Containing Medium

Almost 50 years ago, Grace reported the successful establishment of continuous cell lines from insects that were without defined biological and biophysical parameters (
1
). Since that time, the minimal distinctive parameters for the establishment, maintenance, and manipulation of invertebrate cell cultures have been identified and optimized (
2
,
3
). The establishment of new lines is now routine, and the culture of hundreds of cell lines from a variety of insect species have to date been reported (
2
,
4
,
5
,
6
). During the last 10 years, genetic engineering has revolutionized insect cell culture and baculo virology; this technology has become the method of choice for production of a variety of recombinant proteins of medical and agricultural importance (
7
,
8
). These scientific events have renewed previous interest in using cell culture for production of baculovirus for viral pesticides (
9
–
14
).