Instruction manual for mouse double-stranded RNA (dsRNA) enzyme-linked immunoassay kit

Mouse Double-Stranded RNA (dsRNA) Enzyme-Linked Immunoassay Kit Instructions for Use This kit is for research purposes
only.

Detection range: 96T 3ng/L -180ng/L Purpose of use: This kit is used to determine the content
of double-stranded RNA (dsRNA) in mouse serum, plasma, and related liquid samples.

Experimental Principles This kit uses double antibody sandwich method to determine the level
of mouse double-stranded RNA (dsRNA) in specimens.

The microplate is coated with purified double-stranded RNA (dsRNA) antibodies to make solid-phase antibodies, and double-stranded RNA (dsRNA) is added to the microcellularly coated monoclonal antibodies, and then combined with HRP-labeled antibodies to form an antibody-antigen-antibody complex, which is washed and then added to the substrate TMB coloration
.

TMB is converted to blue under the catalysis of the HRPa enzyme and to a final yellow
color under the action of acid.

The shade of color is positively correlated
with the double-stranded RNA (dsRNA) in the sample.

Absorbance (OD) is determined at a wavelength of 450 nm with a microplate reader and mouse double-stranded RNA (dsRNA) concentrations
in the sample are calculated by standard curves.

ELISA Kit Composition: Kit Composition 48-Well Configuration 96-Well Configuration Preservation Instructions 1 Copy 1 Sealed Film 2 Tablets (48) 2 Tablets (96) Sealed Bag 1 Pcs 1 Microplate Wrapped Plate 1×481× 962-8 °C Preservation Standard: 540 μg/L0.
5 ml×1 bottle 0.
5 ml× 1 bottle 2-8 °C Preservation Standard dilution 1.
5 ml × 1 bottle 1.
5 ml × 1 bottle 2-8 °C Preservation Enzyme reagent 3 ml× 1 bottle 6 ml×1 Bottle 2-8 °C preservation Sample dilution 3 ml×1 bottle 6 ml×1 bottle 2-8 °C preservation Chromogen A solution 3 ml×1 bottle 6 ml × 1 bottle 2-8 °C preservation Chromatographer B solution 3 ml×1 bottle 6 ml × 1 bottle 2-8 °C storage Stop solution 3 ml× 1 bottle 6 ml× 1 bottle 2-8 °C storage Concentrated washing solution (20 ml × 20 times) × 1 bottle (20 ml × 30 times) × 1 bottle 2-8 °C preservation Mouse double-stranded RNA (dsRNA) enzyme-linked immunoassay kit Operation Procedure Summary: CalculationTake the concentration of the standard as the abscissa and the OD value as the ordinate coordinate, draw the standard curve on the coordinate paper, and find the corresponding concentration by the standard curve according to the OD value of the sample; Multiply by the dilution multiple; Or use the concentration of the standard and the OD value to calculate the straight line regression equation of the standard curve, substitute the OD value of the sample into the equation, calculate the sample concentration, and then multiply by the dilution multiple, that is, the actual concentration
of the sample.

Precautions 1.
The kit should be equilibrated at room temperature for 15-30 minutes before use, and if the microplate package is not used after the plate is opened, the plate should be stored in a sealed bag
.

2.
The concentrated washing liquid may have crystallization precipitation, and when diluted, it can be warmed and dissolved in the water bath, and the washing will not affect the result
.

3.
The dosing device should be used for each step of the sample, and the accuracy should be checked frequently to avoid test errors
.

The time of one dosing is controlled within 5 minutes, and if the number of specimens is large, it is recommended to use a platoon gun for dosing
.

4.
Please make a standard curve and do a re-hole
at the same time as each measurement.

If the content of the substance to be measured in the specimen is too high (the sample OD value is greater than the OD value of the standard well), please dilute a certain multiple (n times) with the sample dilution solution before measuring, and multiply the total dilution multiple (× n×5)
when calculating.

5.
Sealing film is only for single use to avoid cross-contamination
.

6.
Please store
the substrate away from light.

7.
Strictly in accordance with the operation of the instructions, the test results must be determined by the microplate reader reading.
8.
All samples, washing liquid and various wastes should be treated
as infectious substances.

9.
The components of different batch numbers of this reagent shall not be mixed
.

Storage conditions and validity period 1.
Kit preservation:; 2-8℃
。
2.
Validity: 6 months