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Albumin Content Kit Instructions
Spectrophotometry 50 Tubes/48 Samples
Spectrophotometry 50 Tubes/48 Samples
Note: Before the formal determination, select 2-3 samples with large expected differences for pre-determination
.
Determination meaning: Determination principle: Self-provided instruments and supplies: Reagent composition and preparation: Sample processing: Determination operation table:
blank tube | standard tube | Assay tube | |
Distilled water (μL) | 10 | ||
Standard (μL) | 10 | ||
Sample (μL) | 10 | ||
Reagent one (μL) | 1000 | 1000 | 1000 |
Mix well, let stand at 25°C for 1min, zero in a 1mL glass cuvette and blank tube, and measure the absorbance value A at 630nm . Denoted as A standard tube and A measuring tube respectively |
Calculation formula: Notes: