Introduction to Ggersin-saline-alkali bacteria

Microbial fermentation technology channel news: China's general microbial species preservation management center senior engineer Xin Yuhua said, Gerts salt-in-nicotine bacteria is 20 years ago by the Chinese Academy of Sciences Microbiology Institute of the elder director Zhou Peixuan researcherfrom Scotland to China, its first separation from Lake Magadi, KenyaAs one of more than 5,000 microorganisms in the center, Gersh salinoid bacteria is dormant either through vacuum freeze-drying, or by using the liquid nitrogen ultra-low temperature freezing method around -190some of thespecies have slept in the freezer for more than half a century, "and when there is demand, they are awakened and put into use." Xin Yuhua saidBut today, 99% of the microbial humans on Earth still don't know how to grow itOnly by being trained do they know what they can do in the future and ultimately make use of them" the species we choose, generally have potential application value and rich biodiversityNow, more and more microorganisms are awakened and in various fieldsXin Yuhua saidThe microbial reserves in the center are accumulated over several generations for a long time, and only time can reflect the value of microbial resource preservationIn addition, microbial resources are at stake in the future of biotechnology innovationnotable that in May, the U.Sannounced the launch of the National Microbiome Program, which will cost the U.Sgovernment $121 million over the next two years and dozens of universities and research institutions to spend $400 million over the next few yearsspecies preservation of the dual "insurance"as China can provide both general bacterial resources services and patented biological materials preservation of the national preservation center, the preservation center in 1995 was included in the "Budapest Treaty International Storage Unit", become an international license of the microbial "bank." " is mainly to collect, preserve, identify, exchange and supply all kinds of biological materialsXin Yuhua told science and technology daily reporter, some of the isolated bacteria in research and development, temporarily do not know what function, as a resource reserve storage, and research topics need to collect, screening of bacteria, after the conclusion of the problem can also be stored backup Over the decades, the Center has provided hundreds of thousands of microbiological strains of various types, and has made outstanding contributions to life science and biotechnology research and development in China walked into the underground storage center, the air filled with silky cool air, and the outdoor sultry contrast In this hundred square meters of liquid nitrogen biological storage tank, filled with more than 30 capacity of 750 liters of "big tank", 53,000 such as bacteria, fungi, yeast and other microbial species hidden in it " almost all of the bacteria are stored in liquid nitrogen, where biological materials are stored and backed up Xin Yuhua lifted a liquid nitrogen tank lid, steamed nitrogen to the surface, the reporter subconsciously backed down Through the mist, a variety of microbial species "sleep" in small iron boxes " liquid nitrogen tank temperature is maintained at around -190, where the bacteria can be preserved for decades Xin Yuhua pointed to the ceiling and said that when liquid nitrogen became less, the "Big Mac" liquid nitrogen canisters upstairs were automatically sensed and added in time, and the expensive "refrigerants" came from Tianjin to the cryogenic freezer, as if into the bank's vault Rows of stainless steel iron cabinets straight to the ceiling, each neatly arranged with a number of "drawer"-like compartments Pull out a "drawer" and you'll see a lot of vacuum small glass tubes with different numbers of bacteria The test tube is equipped with a pale yellow, dry-treated protective agent, hidden in the "sleep" microorganisms, this method of preservation is called vacuum freeze-drying after a double "insurance" to protect the species, a strain of bacteria selling price is only 500-1000 yuan As a public welfare organization, the price of the preservation center is not expensive and the quality is guaranteed It is worth noting that the new microbial species need to be verified and preserved by the preservation center before they can be published in international journals, which is the authority of china's general microbial species conservation center a species of "life and death" cycle at present, the preservation center has a variety of microbial resources 5700 kinds, more than 50,000 strains, for the patent procedure of biological materials have more than 10,000 strains So what has a huge amount of microbial resources, from "wakeup" to being "sleepy", after " bacteria were sent, the survival and purity test was first carried out, and then genetically determined to verify the correctness of the species, and then used vacuum freeze-drying method or liquid nitrogen ultra-low temperature freezing method to preserve the bacteria Xin Yuhua said, when the two methods will be backed up for each other, using liquid nitrogen method to save six copies, vacuum law to back up 12 " the bacteria will be dormant when treated by freezing technology If activated is to be used, it is necessary to activate through the corresponding culture liquid in order to restore the activity Xin Yuhua, taking Gert's saline-in-the-flat-tinbacteria as an example, said that the nutrients needed for the resuscitation bacteria were: 20% sodium chloride, casein, amino acids, pH, etc., and in the appropriate temperature and medium, these sleeping bacteria would be reawakened patent forward but "short board" still exists today, here also preserved the Chinese Academy of Sciences micro-organisms to Chairman Mao's first reishi bacteria species, conducive to women's weight loss of rat Li li lactobacillus, as well as can reduce the field fertilization of the same nitrogen bacteria .. scientists believe that the challenges facing humanity in this day and age, including feeding large populations, producing sustainable energy, protecting the environment or mitigating environmental damage, and human health, are biologically relevant and can be found in the microbial world , more and more strains are found and awakened and applied to people's lives Unlike other intellectual property patents, microbes are the only life form that can be protected by patents, and in the past few years, the annual preservation of patented microorganisms in China has been the world's first , who has worked at the center for 20 years, admits that hardware facilities have improved dramatically since 2000 and now have world-class experimental equipment But reporters in the laboratory saw, such as amino acid analyzers, 35 ultraviolet visible spectroscopic meters and PTP1 temperature control system, Qpix2 automatic colony picking system, denatured high-efficiency liquid chromatography and other essential high-end equipment, are produced in Germany, the United States, Japan; " hardware facilities and preservation technology are international advanced, and the gap abroad is that the diversity of species is not rich enough Xin Yuhua introduced that the United States typical serological preservation center for pure commercial institutions, the bacteria preservation of more comprehensive, more comprehensive application type, the German microbial species preservation center is europe's largest biological resource center, bacterial species preservation is long, and semi-government nature of the Dutch microbial species preservation center, fungal species is the most complete " preservation center on the one hand through various channels to collect new species and new-use strains, on the other hand, also with foreign first-class bacteria center exchange of strains, and thus supplement the rich species Xin Yuhua said that the World Microbiological Data Center has issued the Beijing Declaration on Global Microbial Resource Data Sharing, advocating international cooperation programs on major microbial informationization, and promoting the global microbial resource informationization to a new height