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Isolation of Photosystem II Reaction Center Complexes From Plants

 Last Update: 2020-11-18
 Source: Internet
 Author: User

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Methods to isolate and purify 6- and 5-chlorophyll (Chl) D1/D2/Cyt
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photosystem (PS) II reaction center complexes from plants are presented, and the advantages and disadvantages of each procedure are discussed. One of the simpler 6-Chl procedures and a procedure for isolating 5-Chl complexes are described in detail. Furthermore, a rapid procedure that produces relatively large amounts of less pure 6-Chl material is also described. Criteria to assess the purity of photosystem II (PSII) reaction center preparations are presented, and problems associated with each of the isolation procedures are discussed.

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