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    Home > Medical News > Medical Science News > It is revealed that the DNA of hepatitis B virus and human genome are landscaped

    It is revealed that the DNA of hepatitis B virus and human genome are landscaped

    • Last Update: 2021-01-12
    • Source: Internet
    • Author: User
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    hepatitis B virus (HBV) infection is one of the major public health health problems in the world, and there are currently more people living with chronic hepatitis B virus worldwide. HBV belongs to the hepatophilic DNA virus department, in the human liver cell nucleus can form cccDNA, integrated into the human genome and other viral DNA forms stable existence, which is the key to HBV to maintain long-term chronic infection. Therefore, it is important to study the interaction characteristics between hepatitis B virus and host genome.
    recently, Yang Pengyuan, a researcher at the
    Institute of Biophysics in China, in collaboration with Ji Xiong, a researcher at Peking University's School of Life Sciences, published a research paper entitled 3D Landscape of Hepatitis B virus interactions with human chromatins at Cell Discovery. The study describes the characteristics of the interaction between HBV DNA and the host genome in the form of HBV DNA and integrated forms, providing new potential targets for the treatment of HBV infection and related diseases.
    the study used 3C-high-high-genome-wide sequencing technology, the researchers found in HBV infected HepG2-NTCP cells, HBV ccc The interaction of DNA with the host genome tends to be rich in active enhancers and promoters such as H3K4me3, H3K9ac, H3K4me1, and H3K27ac. In HepAD38 cells that steadily express HBV, it has been found that the integrated form of HBV DNA is capable of forming chromatin loop structures with host genomic DNA and tends to be interoperable with the boot region of the host gene.
    study found that HBV cccDNA interacted with the H3K4me1 region of the host genome, which in turn was modified by chromatin immunoco precipitation (ChIP-qPCR), and found that HBV cccDNA was rich in histone H3K4me1 modification. When the lysine-specific methyl transferase 2C/D (KMT2C/D) occurred in the knock-down cell, HBV RNA transcription was significantly reduced, and the content of HBV surface antigens (HBsAg) and E antigens (HBeAg) secreted in the upper and middle levels was significantly reduced. The results show that HBV cccDNA can be transducted by the host methyl transferase-mediated virus. This study expands academic understanding of the distribution characteristics of HBV in the nucleation of human cells and provides new potential targets for the treatment of HBV infection and related diseases.
    The study was carried out in collaboration with the Institute of Biophysics, Peking University and Beijing Concord Hospital, with Yang Pengyuan and Ji Xiong as co-authors of the paper, Yang Bo, Ph.D. student of the Institute of Biophysics, and Li Boyuan, Ph.D. student of Peking University School of Life Sciences, as co-authors of the paper. The research work has been supported by the Ministry of Science and Technology, the National Natural Science foundation
    the government. (Source: Institute of Biophysics, Chinese Academy
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