Key technologies of the new coronavirus sample killing integration system

New features: Click "Read the original article" in the lower left corner of the text to view the full text of the paper on the mobile phone, log in to the official website www.

Key technologies of the new coronavirus sample killing integration system

ZHANG Dongheyu, GUO Yuntao, ZHANG Liyang, LI Shenwei, WANG Yao, LIU Peipei, WANG Hongqiu, WU Yue, HE Yuping, ZHOU Qun, LUO Haiyun

(Tsinghua University)

DOI：10.

Novel coronavirus pneumonia is an acute respiratory infectious disease caused by the new coronavirus infection, which has posed a threat to the development of the world economy and people's health and safety since the outbreak, and rapid, simple and large-scale testing has played a key role

At present, experts and scholars have preliminarily realized the application of vibrational spectroscopy technology in COVID-19, but there are still two deficiencies: 1) the sample size is small (not more than 300 cases), there is still a big gap with the actual application, and the existing spectral analysis algorithm is not necessarily suitable for large-scale data analysis; 2) The risk of personnel exposure and environmental residue during operation is not considered, and it is urgent to increase disinfection means

Based on previous studies, this paper expands the serum sample size to 857 cases and adds samples with immunological abnormalities as interference terms to verify the specificity

In terms of safety, combined with the research of the author's team in the early stage of discharge plasma sanitize pathogens, this paper developed a sample drying disinfection module and a plasma flexible disinfection film, which was installed on the traditional infrared spectrometer to sanitize protect key parts such as sample placement and instrument operation, so as to realize self-cleaning of the instrument and avoid secondary infection
.

Finally, based on the above spectral detection algorithm upgrade and disinfection module development results, this paper organically combines the sample detection software module and the disinfection hardware module, puts forward the concept of "Investigation and Killing Integration" system, and completes the software and hardware integration and testing
.

1) An integrated system for the detection and killing of samples of pathogens such as novel coronavirus has been established

The "Investigation and Kill Integration" system for new crown samples, as shown in Figure 1, consists of three parts: infrared spectrum new crown rapid detection system, sample drying and disinfection module and flexible disinfection film, which realize the detection and disinfection functions
in the system respectively.

The infrared spectroscopy new crown rapid detection system first obtains the sample spectrum through the FT-IR spectrometer, and enters the data into the identification model based on the PLS-DA or CNN algorithm, and the system quickly gives the detection results
.

During the testing process, two plasma disinfection modules integrated in the system can effectively reduce the spread of virus aerosols in the process and realize the self-cleaning
of the instrument.

The hardware appearance of the integrated system is shown in
Figure 2.

Figure 1 Block diagram of the "Kill-and-Kill" system design

Figure 2 Hardware appearance of the integrated system of Avira and killing

The rapid detection part of the system is developed by collecting 857 serum samples and their FT-IR spectra to form a training database, and introducing PLS-DA and CNN algorithms to establish a spectral recognition classification model, and the modeling steps include serum sample collection, spectral acquisition, spectral data preprocessing, identification model construction and training
.

In the plasma disinfection part of the system, two disinfection modules that are easy to integrate on the spectrometer have been developed according to the actual needs of the site: a sample drying and disinfection module combining a fan, a plasma disinfection unit and a catalytic unit (see Figure 3); The processing of flexible circuit boards to achieve flexible plasma disinfection films with surface media blocking discharge (see Figure 4) effectively improves the safety of the
"Kill-Integrated" system.

Figure 3 Sample drying and disinfection module

Fig.
4 Flexible plasma sterilization film (drive voltage 2.
5kV, 1kHz)

2) Evaluation of the effectiveness of the integrated system for the investigation and killing of samples of pathogens such as novel coronavirus

After the establishment of the integrated system of investigation and killing, the detection or sanitize effectiveness of the infrared spectrum rapid detection system, the sample drying and disinfection module and the flexible disinfection film are evaluated
respectively.

The classification validity of different FT-IR spectral recognition models is evaluated by 10-fold cross-validation method, the sensitivity and specificity of models under different thresholds are calculated, and the ROC curve and statistical prediction accuracy are
plotted.

When only negative and positive samples are distinguished, the binary classification models of partial least squares and neural networks both show good classification effects
.

However, with the addition of the interference term, the detection specificity of the tricategorical neural network model for the positive serum of the new crown was significantly better than that of the partial least squares model, and the ROC curve was shown in
Figure 5.

Figure 5 Summary of the prediction effect of the tri-classification model

Bacteria are used to simulate the contaminated air and surface, and the sterilization effect of the plasma disinfection module is tested
.

Among them, for the sample drying and disinfection module, in the experimental chamber of 1 m³, the killing rate of E.
coli in the form of aerosols in the environment for 30 minutes can reach more than 99.
9%; For flexible disinfection films, working for 5 min can achieve an average killing log of E.
coli on its surface greater than 3
.

Both modules meet the requirements
of the 2002 edition of the Sterilization Technical Specification.

Based on the FT-IR spectroscopy database of new coronavirus serum samples, develop models and software for the rapid detection of pathogen samples such as the new coronavirus, design two plasma sanitize modules for sanitize detection sites and instruments, and finally combine the above software and hardware modules with traditional infrared spectrometers to establish a "integrated killing" system for new coronavirus samples and complete the preliminary test
of the prototype.

In terms of "checking", the sensitivity and specificity of the new crown antibody prediction model can reach more than
96%.

In terms of "killing", the killing rate of E.
coli in the simulated disinfection test of the sample drying and disinfection module and the flexible disinfection film can reach more than 99.
9%, which meets the requirements
of the disinfection effect in the 2002 edition of the "Disinfection Technical Specification".

Compared with other technologies at home and abroad that rapidly detect new coronavirus samples based on spectral methods, the system has formed a significant improvement in sample size and test safety, expanding the scope of application of spectral detection methods, and is expected to become a powerful supplement
to the current new crown detection methods.

At the same time, the technology is expected to be applied to the detection of other kinds of viral infections or immune diseases, unleashing the greater potential
of spectral methods in medicine and public health.

Citation information

Zhang Dongheyu,Guo Yuntao,Zhang Liyang,et al.
Key Technologies of Integrated System for Investigation and Killing of Novel Coronavirus Samples[J].
Proceedings of the CSEE,2022,42(12):4623-4632.

ZHANG Dongheyu, GUO Yuntao, ZHANG Liyang, et al.
Key Technologies of Integrated System for the Detection and Disinfection of COVID-19 Samples [J].
Proceedings of the CSEE，2022，42(12)：4623-4632(in Chinese).

Zhang Dongheyu (1999), female, doctoral candidate, the main research direction is gas discharge plasma, vibration spectroscopy analysis, z_d_h_y@163.
com

Haiyun Luo (1982), male, Ph.
D.
, associate professor, the main research field is the medical application of discharge plasma, lhy@tsinghua.
edu.
cn

Zhou Qun (1969), female, Ph.
D.
, associate professor, main research direction is vibrational spectroscopic analysis of complex mixture systems, zhouqun@tsinghua.
edu.
cn

Editor-in-charge: Li Jingyan

Review: Linlin Hu

statement

This article is an original work, the text and picture copyright involved belong to the editorial department of the Proceedings of the CSEE, according to the latest regulations of the National Copyright Administration, print media, website, Weibo, WeChat public account reprint, excerpt and edit the works of my editorial department, please contact my editorial department
in advance.

Personally, please follow the original text of this WeChat to forward and share