Labeling of Oligonucleotides with Fluorescein
-
Last Update: 2020-12-08
-
Source: Internet
-
Author: User
Search more information of high quality chemicals, good prices and reliable suppliers, visit
www.echemi.com
Oligonucleotides are a convenient alternative to conventional, cloned
DNA
probes since they can be rapidly synthesized in large quantities relatively cheaply. They can be made to very specific regions of the genome, without reference to restriction enzyme sites, and are therefore of particular value in the analysis of gene families where sequences may be highly conserved. Oligonucleotides are stable, do not self-anneal, and generally cause fewer problems with nonspecific binding. They are amenable to several labeling techniques, both radioactive and nonradioactive; such methods include chemical modification during synthesis and the enzymatic incorporation of labeled nucleotides at the 5′ end or the 3′ end.
This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only.
This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of
the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed
description of the concern or complaint, to
service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content
will be removed immediately.
