Large DNA Transformation in Plants

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Large DNA Transformation in Plants

 Last Update: 2021-02-14

 Source: Internet

 Author: User

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Large
DNA
(>100 kb) transformation methods have been reported in tomato using
Agrobacterium
-mediated transformation (
1
) and tobacco using biolistic bombardment (
2
). Both methods used modified bacterial artificial chromosomes (BACs) such as BIBAC or pBACwich. BIBAC vector (used in tomato) is capable of transferring large DNA fragments from
Agrobacterium
into plants. pBACwich (used in tobacco) utilizes a Cre/
lox
site-specific recombination system to integrate large DNA fragments into plant chromosomes.

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Large DNA Transformation in Plants

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