Layer-analise Technology

ion exchange layering technology is based on ion exchange cellulose or ion exchange glucosaccharide gel as a fixed phase, toproteinsamples are a technique for moving phases, separating and purification of proteins,nucleic acids, enzymes, ef and polysaccharides.

(i) principle

in cellulose and glucosin molecules combined with a certain ion base group, when combined with cation groups, can be exchanged for ions. Such as diacetaminoethylene (Dicthylaminoethyl, DEAE) cellulose.

binds DEEAE to cellulose and contains positively charged cation cellulose-O-C6H14 N-H, whose anti-ions are anions (e.g. Cl-, etc.) that can be exchanged with negatively charged protein anions. When combined with anion groups, cations can be replaced, called cation exchangers, such as carboxymethy (CM) cellulose. A negatively charged anion (cellulose-O-CH2-COO-) on a cellulose molecule with an anti-ion of cations (e.g. Na-plus, etc.) that can be exchanged with the positively charged protein cation.

solution is the same as the protein isoelectre, the static charge is 0, and when the solution pH is greater than the protein isoelectre, the pH of the solution is greater than the protein isoelectre, then the base is free, and the protein is negatively charged.

On the other hand, when the pH of the solution is less than the isoelectre of the protein, the amino ion is ion, and the protein is positively charged. The farther the pH of the solution is from the protein's iso-point, the more charge the protein has. On the other hand, there are fewer.serum proteins are negatively charged, but the degree of negative charge varies from protein to albumin, followed by globulin, globulin and globulin.

At the appropriate salt concentration, when the pH of the solution is higher than the isoelectronic point, the protein is adsorbed by an anion exchanger, and when the pH of the solution is lower than the isoelectronic point, the protein is adsorbed by the cation exchanger. Because of the different charges that come with various proteins. They also bind to different levels of the exchanger, as long as the solution pH changes, it will directly affect the adsorption of proteins and exchange agents, which may separate different proteins one by one.

exchanger has the ability to exchange adsorption for both collome ions (e.g. proteins) and inorance salt ions (e.g. NaCl), which produce competitive exchange adsorption when both are present in a layering process. When the concentration of Cl-is large, the protein is not easy to be adsorbed, after adsorption is also easy to be washed away, when the Cl-concentration hours, the protein is easy to be adsorbed, adsorption is not easy to be enchanted.