Masson dyes.

Masson staining, used to display one of the fiber dyeing methods.reagents:​Regaud is Sumu: Sumu essence 1g, 95% alcohol 10ml, glycelin 10ml, distilled water 80ml.​add sumu essence to distilled water to heat dissolve, cool and add alcohol and glycelin, put a few days can be applied.Masson Lichun red acid complex: Lichun red 0.7g, acid red 0.3g, distilled water 99ml, ice acetic acid 1ml.0.2% ice acetic acid aqueous solution: ice acetic acid 0.2 ml, distilled water 100 ml.1% hydrolytic solution: phosphate 1g, distilled water 100 ml.aniline blue water solution: aniline blue 2g, distilled water 98 ml, ice acetic acid 2 ml.1% light green water solution: light green 1g, distilled water 100 ml.Masson tricolor step:​1. Paraffinslice dewax to water.​2. Chromium treatment or precipitation of mercury salts (formaldehyde-fixed tissue can be slightly more so).​3. Water and distilled water are washed in turn.​4. Dye the nucleus 5-10min with Regaud Sumu Semen or Weigert Sumu Semen.​5. Adequate washing, such as over-stained alcoholic hydrochloric acid differentiation.​6. Distilled water wash.​7. Use Masson Lichun red acid compound liquid 5-10min.​8. Wash for a moment with 2% ice acetic acid aqueous solution.​9. 1% phosphate water solution differentiation 3-5min.​10. Without washing, dye 5min directly with aniline blue or light green liquid.​11. Wash for a moment with 0.2% ice acetic acid aqueous solution.​12. 95% alcohol, waterless alcohol, xylene transparent, neutral gum seal.results: collagen fibers, mucus, cartilage blue (e.g. photogreen dye is green), cell pulp, muscle, cellulose, glial red, cell nucleus black blue.experience and description:​1. Control the dyeing steps.​2. Tissue fixation plays an important role, and dyeing time can be extended or shortened depending on the fixation fluid.​3. 0.2% ice acetic acid water solution wash, can make the tone clear and bright.​4. The hydrolytic solution of phosphate has differentiation effect on Lichun red and acidic redness, which is controlled under the mirror, muscle fiber and cellulose are red, and collagen fiber is pale pink.