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Measurement of Aldehyde Formation as an Assay for Polyamine Oxidase Activity

 Last Update: 2020-12-21
 Source: Internet
 Author: User

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1 2 dichloroethene

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The polyamines spermine and spermidine can be metabolized by polyamine oxidases in two ways, oxidative determination or oxidative cleavage (
1
,
2
) (
see
Chapter 1 ). An example of the former is the amine oxidase present in bovine (and other ruminant) sera (
3
) that deaminates polyamines in accordance with Fig. 1. In contrast, tissue polyamine oxidase (typified by rat liver polyamine oxidase) (
4
) cleaves spermine or spermidine with the release of an aminopropyl moiety (Fig. 2 ).Fig. 1.
Oxidation of spermine or spermidine by bovine serum amine oxidase.
Fig. 2.
Oxidation of spermine or spermidine by rat liver polyamine oxidase.

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